Aerial Parts Research Articles

GC/MS chemical profiling and isolation of secondary metabolites from Leontice ewersmannii.

GC/MS chemical profiling of the aerial parts of Leontice ewersmannii revealed the presence of various bioactive compounds of which 20 compounds were in n-hexane fraction and 3 compounds in basic DCM fraction. Among the identified compounds alkaloids, fatty acids and their esters, fatty alcohols, hydrocarbons, and sterol weredetected. Phytochemical investigation of the aerial parts of L.ewersmannii led to isolation of 17 compounds including 1-heptacosanol (1), phytol (2), lupenone (3), palmitic acid (4), oleic acid (5), stigmasterol (6), stigmast-7-en-3-ol (7), methyl palmitate (8), methyl linoleate (9), butyl palmitate (10), butyl linoleate (11), dimethyl dodecanedioate (12), methyl cerebronate (13), β-sitosterol (14), β-sitosterol 3-O-β-glucopyranoside (15), sophoridine (16) and camoensine (17). The structures of the isolated compounds were determined by 1D NMR, HR-ESI-MS, and GC-MS. All methyl esters of fatty acids are possible artifacts during extraction. Additionally, the antimicrobial activity of the total extract and different fractions was screened, and they exhibited no inhibition of the growth at the tested concentration ranging from 8 to 200 μg/mL.

Introducing the glycyrrhizic acid and glabridin rich genotypes from the cultivated Iranian licorice (Glycyrrhiza glabra L.) populations to exploit in production systems

Currently, the stable, uniform, and highly efficient production of raw materials for pharmaceutical companies has received special attention. To meet these criteria and reduce harvesting pressure on the natural habitats of licorice (Glycyrrhiza glabra L.), cultivation of this valuable plant is inevitable. In the present study, to introduce the glycyrrhizic acid (GA)- and glabridin-rich genotypes from cultivated Iranian licorice, forty genotypes from eight high-potential wild populations were cultivated and evaluated under the same environmental conditions. The GA content varied from 5.00 ± 0.04 mg/g DW (TF2 genotype) to 23.13 ± 0.02 mg/g DW (I5 genotype). The highest and lowest glabridin content were found in the K2 (0.72 ± 0.021 mg/g DW) and M5 (0.02 ± 0.002 mg/g DW) genotypes, respectively. The rutin content in the leaves of the studied genotypes varied from 1.27 ± 0.02 mg/g DW in E4 to 3.24 ± 0.02 mg/g DW in BO5 genotypes. The genotypes from the Ilam population were characterized by higher vegetative growth and yield traits in the aerial parts and roots. The average root dry yield was 2.44 tons per hectare (t/ha) among the studied genotypes and a genotype from Ilam (I5) yielded the maximum value (3.08 ± 0.034 t/ha). The highest coefficient of variation among the genotypes was observed for leaf width (CV = 34.9%). The GA and glabridin-rich genotypes introduced in this study can be used in the future breeding programs to release new bred licorice cultivars.

Crotonoids A-H, Labdane-Type Diterpenoids with Anti-Inflammatory Activity from Croton sublyratus.

Croton sublyratus (Euphorbiaceae) is a traditional medicinal plant used by the Thai populace to treat helminthic infections and dermatologic conditions. In present study, eight new labdane-type diterpenoids, crotonoids A-H (1-8) and one known analogue (9) were isolated from the aerial parts of C. sublyratus. Compounds 6 and 7 belong to the rare class of 14,15-dinor-labdane diterpenoids. Compound 8 exhibited a rare 14,15,17-trinor-labdane skeleton. The structures of all these diterpenoids were elucidated by spectroscopic data analysis, electronic circular dichroism calculations, and single-crystal X-ray diffraction analysis. Compound 9 exhibited moderate anti-inflammatory activity via the inhibition of NO production in lipopolysaccharide-induced RAW 264.7 cells.

Flavonoids From the Aerial Parts of Sophora tonkinensis and Their Potential Anti-Inflammatory Activities.

Four undescribed prenylated flavonoids, sophoratones A-D (1-4), and 17 known flavonoids, were obtained from the aerial parts of Sophora tonkinensis. Their structures with absolute configurations were elucidated by detailed interpretation of NMR spectroscopy, mass spectrometry, and ECD calculations. Meanwhile, the ability of these compounds to inhibit the release of nitric oxide (NO) by a lipopolysaccharide induced mouse in RAW 264.7 cells was assayed. The results indicated that some compounds exhibited clear inhibitory effects, with IC50 ranging from 19.91±1.08 to 35.72±2.92 μM. These results suggest that prenylated flavonoids from the aerial parts of S. tonkinensis could potentially be used as a latent source of anti-inflammatory agents.

Morphological characterisation of ethnomedicinally important Kaempferia L. (Zingiberaceae) and its present distribution status in Manipur, North-East India

Kaempferia L., a member of the Zingiberaceae family, is one of the most potent medicinal plants in India and requires proper attention. Our efforts to collect various species of Kaempferia in their natural habitat led to the collection of 30 accessions, including K. galanga, K. marginata, K. parviflora, K. rotunda, and five other unidentified Kaempferia species that are likely natural hybrids with no distinguishing features. It has been found that rhizomes are the most usable portion of the plant, and people in this region use them the most for medical purposes. The present study aimed to describe and evaluate the morphological structures of the plant, incorporating 52 phenotypic descriptors. Descriptive statistics and multivariate analysis were employed to explore diversity. The majority of the traits tested were highly variable. The elevation range (28704.02%) and plant height (at 648.03 %) had the largest coefficients of variation (CV %), whereas anther length (0.06 %) and corolla shape (0.19 %) had the lowest. Rhizome size has a high positive correlation with the odour of the aerial plant parts, growing, dormant, new shoots sprouting, and flowering seasons. However, it shows negative correlations with elevation, lifespan of the flower, and number of leaves. Principal component analysis (PCA), revealed that the first five components accounted for 89.90 % of the variability, and hierarchical grouping based on accession dissimilarity revealed two significant groupings. It was found that wild K. species populations were genetically diverse, suggesting that this diversity can be used for agronomic and horticultural purposes to isolate and characterise numerous important, well-adapted genotypes with the potential to significantly increase yields. Therefore, it is crucial to conserve the genetic material that is currently available.

IN VIVO ACUTE ORAL TOXICITY AND ANTI-INFLAMMATORY EFFECTS OF PLUCHEA PTEROPODA ETHANOL EXTRACT

Pluchea pteropoda, an annual plant in the Asteraceae family, has traditionally been used in Vietnamese folk medicine as an alternative treatment for high fever, indigestion, and painful and swollen wounds. The present study investigates the acute toxicity and antiinflammatory effects of P. pteropoda aerial parts ethanol extract (EtPP) in animal models. EtPP exhibited no toxicity in mice with a single dose of up to 5000 mg/kg. EtPP significantly inhibited the carrageenan-induced edema paw in rats at 77.5 and 150.0 mg/kg body weight doses. The strongest inflammation inhibition effect (37.4%) was recorded at a dose level of 150.0 mg/kg after 3 h after carrageenan administration.

Phytochemical profile and antimicrobial, anti-inflammatory, antioxidant activities of the essential oils in the aerial part and root of Glehnia littoralis Fr. Schmidt ex Miq

The root of Glehnia littoralis is used as a good traditional Chinese medicine. The aerial part of G. littoralis is often discarded as waste in China, although it is very abundant, which results in waste of resources. In order to utilize the resources, the essential oils of the aerial part and root were isolated from G. littoralis, separately named as GLA and GLR. Their phytochemical profile, antimicrobial, anti-inflammatory and antioxidant effects were investigated. The results showed that the concentrations of GLA and GLR were separately 0.933 g/kg and 1.365 g/kg, and nineteen components were identified from both oils. Among them, panaxynol was the common component with a content of 38.21 % in GLA and 74.02 % in GLR. GLA potently inhibited F. solani and F. incarnatum, GLR strongly suppressed S. albus. GLA and GLR reduced the production of NO, IL-1β, IL‐6, TNF-α in LPS-stimulated RAW 264.7 macrophages. This study provided a theoretical basis for the utilization of the aboveground parts of G. littoralis.

Assessment of the content of biologically active substances in Hypericum perforatum along an altitude gradient

An assessment of the content of flavonoids in the herb St. John’s wort, selected in natural phytocoenosis along an altitudinal gradient, is presented. We used generally accepted methods described in the relevant manuals when conducting research. The material for the study was air-dried samples of the aerial parts of St. John’s wort (Hypericum perforatum) collected at different altitudes. The features of the accumulation of flavonoids (leucoanthocyanins, catechins, flavonols) and the amount of anthracene derivatives in the herb St. John’s wort (Hypericum perforatum) in the phase of mass flowering along an altitudinal gradient were studied. It has been shown that an increased level of anthracene derivatives (in terms of hypericin) is observed at altitudes of 615 - 716 m above sea level. The amount of anthracene derivatives (in terms of hypericin) significantly decreases along the altitudinal gradient. The content of catechins in samples taken along the altitudinal gradient varies within 0.17 - 0.36 mg%. It has been established that plants selected at different altitudes from 615 to 2025 m above sea level are characterized by other content of flavonoids. The samples in the altitude range 615 - 716 m above sea level have the maximum content. The plants selected in the vicinity of the village had the best characteristics for the accumulation of the mass fraction of flavonols. Makhchesk, Irafsky district of the Republic of North Ossetia - Alania, in coenopopulations of a cereal-forb meadow at 1340 m above sea level. Thickets of Hypericum perforatum are found on slopes of southern and southeastern exposure.

Use of Phosphite Preparations to Protect Ash Seedlings Fraxinus excelsior L. against Phytophthora spp. and Hymenoscyphus fraxineus Pathogens

In this study, greenhouse tests were conducted on 240 Fraxinus excelsior seedlings to investigate the simultaneous damage caused by thea pathogenic fungus and oomycetes. The experiment was performed under controlled conditions in the greenhouse of the Institute of Forest Research in Sękocin Stary (Poland). Three species of oomycetes were used for the experiment: Phytophthora plurivora, Phytophthora taxon hungarica, Phytophthora megasperma, and the fungus Hymenoscyphus fraxineus. Inoculations using the fungus were carried out on shoots and in plant pots in which the soil was mixed with the three Phytophthora species mentioned above, both simultaneously and separately, which made it possible to recognize the cumulative effect of the related plant infection. The aim of the study was to investigate the effect of phosphite-containing preparations on the health of common ash under conditions of threat to the roots by Phytophthora spp. and damage to the aerial parts of the plant by the fungus, as well as the possible occurrence of synergistic effects. Two types of protective preparations (Actifos and Phos60 of the nitrogen and potassium forms, respectively) were used. It was found that the inoculation of ash seedlings with the fungus H. fraxineus resulted in plant mortality, while the mixture of Phytophthora did not cause significant damage. It was confirmed that when pathogens coexist, a phenomenon occurs that leads to an acceleration in the development of disease symptoms and, thus, to plant mortality. In vitro tests confirmed the usefulness of phosphite preparations for the protection of ash seedlings.

An efficient determination of phenolic compounds by HPLC-DAD and their bioactivity assay from aerial parts of Eucalyptus tereticornis plant

An efficient determination of phenolic compounds by HPLC-DAD and their bioactivity assay from aerial parts of Eucalyptus tereticornis plant

Chemical Composition, Antioxidant Activity, and Milk-Clotting Activity of Aqueous Extracts from Leaves, Stems, and Flowers of Three Tunisian Ecotypes of Spontaneous and Cultivated Onopordum nervosum ssp. platylepis Murb.: A Potential Novel Vegetable Rennet Option

This study aimed to compare the chemical composition of aqueous extracts from different aerial plant parts (leaves, stems, and flowers) of Onopordum nervosum ssp. platylepis growing in different regions (Sousse, Kairouan, and Nabeul) in Tunisia, as well as their antioxidant and milk-clotting properties for both spontaneously grown and cultivated plants. Results showed that phenolic composition varies significantly among ecotypes and plant organs (p < 0.05), with flowers containing the highest amounts of total phenols and flavonoids in both plant types. The flowers from Nabeul (NA) region showed the highest amounts of total phenols and higher phenolic contents compared to leaves and stems, with 44.75 mg GAE/g and 39.79 mg GAE/g in spontaneous and cultivated plants, respectively. However, flowers of spontaneously plants grown in Sousse (SO) showed the highest total flavonoid contents (11.42 mg QE/g). Additionally, the findings indicated that flowers contained higher concentrations of mono- and disaccharides than leaves and stems. The antioxidant activity showed that the radical scavenging activity of O. platylepis aqueous extracts is significantly affected by the organ and genotype (p < 0.05). NA genotype revealed the highest potency in inhibiting free radicals, with flowers having the lowest IC50s values in 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) tests, registering 0.13 ± 0.02 and 0.14 ± 0.01 mg/mL, respectively. Milk-clotting activity was limited to flowers of this thistle. Moreover, SO and NA genotypes were identified as the most potent populations to coagulate milk in spontaneous and cultivated plants. The principal component analysis confirms the organ and genotype variability in this thistle, with the first two axes explaining 64.15% of the variance and highlighting a distinct flower group. The obtained results suggest that the domestication of this thistle could be useful for the conservation of biodiversity and the promotion of genotypes with potential technological properties.

Investigation of Antimicrobial Activity of Iron Oxide Nanoparticles (FeONPs) and Zinc Oxide Nanoparticles (ZnONPs) Produced with Aerial Part Extract of Cucumber

Investigation of Antimicrobial Activity of Iron Oxide Nanoparticles (FeONPs) and Zinc Oxide Nanoparticles (ZnONPs) Produced with Aerial Part Extract of Cucumber

Chemical Constituents of the Artemisia ciniformis Aerial Parts Grown in the Northeast of Iran and Their Chemotaxonomic Significance

Background: The Artemisia genus is one of the largest genera of the Asteraceae family, with various traditional and modern reported uses. Within this group, Artemisia ciniformis (Dermane Talaei) is an endemic plant of Iran with antioxidant, antimalarial, anticancer, cytoprotective, and neuroprotective properties. Due to these potential benefits, further phytochemical research needs to be conducted to understand its chemotaxonomic and pharmaceutical effects. Objectives: The main purpose of this study is to investigate the dichloromethane fraction of A. ciniformis from Iran for phytochemical and chemotaxonomic aspects. Methods: Artemisia ciniformis aerial parts were collected, dried, and extracted by the maceration method using dichloromethane after defatting. The prepared extract was subjected to chromatographic techniques, including column chromatography, vacuum liquid chromatography, and HPLC, to isolate purified compounds, which were identified using spectroscopic analyses such as NMR and EI-MS. Results: Two tetrahydrofuran-type sesquiterpenoids: (1) Hydroxydavanone and (2) arteincultone; two acetophenone derivatives: (3) Xanthoxylin and (4) 2,4-dihydroxy-6-methoxy acetophenone; and a flavonoid, (5) jaceosidin, were isolated. Additionally, a polymethoxylated flavone was obtained in small amounts, and its structure was tentatively assigned as (6) 5,6-dihydroxy-2-(3-hydroxy-4,5-dimethoxyphenyl)-7-methoxy-4H-chromen-4-one (Ciniformon). Conclusions: Artemisia ciniformis is a rich source of phytochemicals from various categories, such as sesquiterpenes and phenolic compounds, that can be used in systematic studies and medicinal research to treat human disorders by discovering new biologically active as well as lead compounds.

Anthelminthic and antimicrobial effects of hedge woundwort (Stachys sylvatica L.) growing in Southern Kazakhstan

Anthelminthic and antimicrobial effects of hedge woundwort (Stachys sylvatica L.) growing in Southern Kazakhstan

Chemical analysis and antibacterial activity of the Achillea millefolium extract

Achillea millefolium L., a traditional medicinal plant from the Asteraceae family, is known for its anti-inflammatory, anti-ulcer, and anti-cancer properties, attributed to its rich content of phytochemicals (such as flavonoids, alkaloids, ter¬penes, tannins, and phenolic acids). This study has focused on the chemical analysis of the oil extracted from the aerial parts of Achillea millefolium subsp. millefolium, cultivated and harvested in Kurdistan, Iraq. The oil was extracted by using a Clevenger apparatus for hydro-distillation and was analysed by using gas chromatography with a flame ionization detector and an HP-5 MS capillary column. The analysis revealed the presence of 1,8-cineole (eucalyptus oil), Artemisia ketone, camphor, linalyl acetate, and D-limonene, with Artemisia ketone having the highest concentra¬tion at 15.04% and D-limonene the lowest at 7.51%. The extract of Achillea millefolium has been shown to aid in the treatment of oral mucositis (a common side effect of anticancer chemotherapy), likely due to the flavonoids and tannins present in the plant.

Chemoinformatic study on phytochemicals from Melissa officinalis for ligand based drug design inhibition of aflatoxins synthesis

This study involved extracting 2D structures of fifty-one (51) GC-MS chromatogram-generated compounds from PubChem a free depository database for generating a library of compounds and obtaining the 3D structure of polyketide synthase A, an enzyme recognized for the biosynthesis of aflatoxin in Aspergillus species, from the protein data bank. In silico (molecular docking was performed to assess the interactions of the phytochemicals in the sample and were screened for their inhibitory potential, having higher binding energy, and favorable pharmacokinetics profiles. The docking analysis outcome indicates that nine (9) compounds exhibited binding energies ranging from −11.314 to −8.365 kcal/mol which is higher than the control (PLM) with a binding energy of −8.131 kcal/mol. It is observed that many of the compounds interacted with the target using hydrophobic interaction and only a few added hydrogen bonds and salt bridges. This research has shown a potential of the aerial part of Melissa officinalis vegetable for drug development to inhibit the synthesis of aflatoxins from Aspergillus flavus. The study has showcased good scientific information for the development of natural products for the prevention and treatment of aflatoxin contamination in agricultural products.

Sodium Chloride Tolerance during Germination and Seedling Stages of Tomato (Solanum lycopersicum L.) Lines Native to Mexico

Tomato is considered moderately sensitive to salinity, which detracts from the quality and yield of its fruit; therefore, wild populations have been used as a genetic resource. The aim of this research was to identify lines derived from wild tomato populations with tolerance to salinity during the germination and seedling stages. During germination, 52 wild lines and 2 commercial hybrids (Imperial®, Reserva®) were subjected to treatment with 150 mM and 0 mM NaCl and evaluated. The test was carried out for 20 days in a germination chamber with constant darkness, a temperature of 25 ± 2 °C and relative humidity conditions of 80 ± 4%. At the seedling stage, 22 wild tomato lines with the best performance in the germination test and 2 commercial hybrids (Imperial®, Topanga®) were evaluated for 12 days in a floating raft system. Concentrations of 175 mM and 0 mM of NaCl were used. During germination, the saline condition decreased the germination percentage (65.2%), speed of germination (88.2%), steam length (72.5%), root length (46.56%), number of normal plants (59.5%), stem dry matter (68.78%), root dry matter (61.99%), and total dry matter (67.1%). At the seedling stage, this condition decreased (p < 0.05) the aerial part dry matter (46.37%), leaf area (59.35%), root length (42.43%), final plant height (40.24%), and growth rate (71.42%). Seventeen tolerant genotypes were identified in one of the two developmental stages, while one genotype showed tolerance in both stages. These results indicate that there are different response mechanisms in each developmental stage. Native tomatoes play an important role in the identification of tolerant genotypes since they can be used as genetic resources for obtaining commercial genotypes with salt tolerance.

Antiurolithiatic activity of Indian medicinal plant: Ocimum kilimandscharicum Gurke (Lamiaceae)

Urolithiasis is the most prevalent condition of the urinary system, characterized by the formation of stones inside the urinary tract. It is urgent to look for a natural urolithiasis therapy due to the serious side effects of conventional medications. Hydro-alcoholic (80% v/v) extract of the aerial parts of Ocimum kilimandscharicum (OK) and its ethyl acetate, chloroform, n-butanol, aqueous, and n-hexane fractions were subjected to in vitro antiurolithiatic screening as well as preliminary screening of phytochemicals. The in vitro antiurolithiatic activity of O. kilimandscharicum was studied using its hydroalcoholic extract (HAEOK). Calcium phosphate test using a colorimetric approach and calcium oxalate assay using a titrimetric model were used to determine the proportion of calcium oxalate crystals that dissolved. Total phenolic content (TPC) and total flavonoid content (TFC) were measured for the extract and fractions of OK. Ethyl acetate fraction (EAFOK) had a greater capacity to suppress crystal formation in both the calcium phosphate and calcium oxalate assays. The percent dissolution of calcium oxalate by HAEOK and EAFOK (31.48 ± 0.920% and 39.21 ± 0.903%) and calcium phosphate crystals by HAEOK and EAFOK (59.03 ± 0.820% and 66.62 ± 0.468%) was determined, respectively. At p < 0.05 and p < 0.01, differences between the results were regarded as significant. Cystone was employed as a standard drug. This study revealed that EAFOK showed significant antiurolithiatic activity. The antiurolithiatic activity of the extract/fraction was attributed to the steroids, triterpenoids, and flavonoid content of OK.

Uptake and translocation of pharmaceutically active compounds by olive tree (Olea europaea L.) irrigated with treated municipal wastewater

IntroductionThe use of treated municipal wastewater (TWW) represents a relevant opportunity for irrigation of agricultural crops in semi-arid regions to counter the increasing water scarcity. Pharmaceutically active compounds (PhACs) are often detected in treated wastewater, posing a risk to humans and the environment. PhACs can accumulate in soils and translocate into different plant tissues, reaching, in some cases, edible organs and entering the food chain.MethodsThis study evaluated the uptake and translocation processes of 10 PhACs by olive trees irrigated with TWW, investigating their accumulation in different plant organs. The experiment was conducted in southern Italy, in 2-year-old plants irrigated with three different types of water: freshwater (FW), TWW spiked with 10 PhACs at a concentration of 200 µg L−1 (1× TWW), and at a triple dose (3× TWW), from July to October 2021. The concentration of PhACs in soil and plant organs was assessed, collecting samples of root, stem, shoot, leaf, fruit, and kernel at 0 (T0), 50 (T1), and 107 (T2) days of irrigation. PhACs extraction from soil and plant organs was carried out using the QuEChERS method, and their concentrations were determined by high-resolution mass spectrometry coupled with liquid chromatography.ResultsResults of uptake factors (UF) showed a different behavior between compounds according to their physicochemical properties, highlighting PhACs accumulation and translocation in different plant organs (also edible part) in 1× TWW and 3× TWW compared to FW. Two PhACs, carbamazepine and fluconazole, showed interactions with the soil–plant system, translocating also in the aerial part of the plant, with a translocation factor (TF) greater than 1, which indicates high root-to-leaf translocation.DiscussionFindings highlight that only few PhACs among the selected compounds can be uptaken by woody plants and accumulated in edible parts at low concentration. No effects of PhACs exposure on plant growth have been detected. Despite the attention to be paid to the few compounds that translocate into edible organs, these results are promising for adapting wastewater irrigation in crops. Increasing knowledge about PhACs behavior in woody plants can be important for developing optimized wastewater irrigation and soil management strategies to reduce PhACs accumulation and translocation in plants.

Anti-diabetic potential of Chamaerops humilis L. aerial parts: phenolic compounds with α-amylase and α-glucosidase inhibitory activates in-vitro, in-vivo and in-silico studies

Five compounds were isolated from the aerial parts of Chamaerops humilis L. and characterized as tricin-7-rutinoside (1), tricin (2), dihydrotricin (3) diasteroisomer of tricin 4’-O(erythro-β-guaiacyl glyceryl) ether (salcolin A)(4a), tricin-4’-O-(threo-β-guaiacyl glyceryl) ether (salcolin B) (4b) and (-)-(5s, 6s)-5,6-dihydro-3,8,10-trihydroxy-5-(4-hydroxy-3-methoxyphenyl)-6-hydroxymethyl-2,4-dimethoxy 7H benzo [e] xanthen-7-one (5). Their structures were elucidated by comparing their spectral data with those reported in the literature. To the best of our knowledge, compounds (3-5) were reported here for the first time from Chamaerops humilis. The methanolic extract of Chamaerops humilis (CHME) and the five isolated compounds were studied for its anti-diabetic effect using in vitro α-glucosidase and α-amylase inhibitory assay that showed a significant activity. The toxicological study indicated that CHME was safe up to 5000 mg/kg bw. Two doses were selected for the in vivo study (800 and 1600 mg/kg bw) and orally administered to high fat diet/streptozotocin-induced diabetic rats for 10 consecutive days. The results revealed that the CHME improved the blood glucose levels in a dose dependent manner, the dose 1600 mg/kg showed the best results dependently along the period of the experiment. CHME and the five isolated compounds were evaluated in silico for their anti-diabetic effect. The anti-inflammatory effect of CHME (1600 mg/kg bw) was proved by significant downregulation of expression for interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and matrix metalloproteinase-1 (MMP-1) genes. In conclusion, CHME and its constituents may be a potential hypoglycemic agent working through the anti-inflammatory and enzyme inhibitory pathways. The docking studies demonstrated that compounds Tricin7-rutinoside and Salcolin isomers had promising binding energies of -9.10 kcal/mol and -8.70 kcal/mol, respectively, against α-amylase. These binding energies were superior to that of acarbose, which had a binding energy of -7.60 kcal/mol. In addition, docking analysis provided promising results for the inhibition of α-glucosidase by Tricin7-rutinoside and Dihydrotricin with binding affinity -7.9 kcal/mol and -7.9 kcal/mol, respectively while the binding affinity of acarbose is -6.40 kcal/mol. Overall, the methanolic extract from Chamaerops humilis exhibited good in silico of α-amylase and α-glucosidase enzyme inhibition potential. These findings suggest that these compounds may have potential as anti-diabetic agents, with further research needed to verify their efficacy and safety.